This research consists of two primary objectives: identifying opportunities, information gaps, existing resources and research needs by engaging non-government organizations, the California Department of Transportation, and the California Department of Fish and Wildlife biologists who expressed interest in working on this project; and with input from these biologists, conduct a preliminary investigation for at least 10 sites, each with concrete median barriers, cable guards or thrie-beam barriers, and vegetated/developed medians, for comparison of rates of wildlife-vehicle collisions with large and all wildlife, as indicators of wildlife connectivity.